Care was taken to select only young rats with good health, with the assignment of the rats to their respective control groups as well as treatment groups being random. Their cages were specially arranged in a way to reduce cage placement effects. The animals underwent a unique identification and later confined in their different cages for five days prior to the commencement of this study to enable the animals to acclimatize to the conditions in the laboratory.
The compound for the test was given by gavages through drinking water, where the test substances dissolved in the water.
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