After marking the mid-point of the test tube, â€œconditionedâ€ water i.e. water that had been standing in an open container for 24 hours to allow escape of chlorine was placed in the test tube but enough room was left for the stopper. A live planarian (DugesiaÂ sp.) was then placed in the middle of a test tube using a pipette and a stopper was placed on one side of the test tube. With the stoppered end facing upwards, the test tube was placed in a holder in a position that allowed even illumination of the test tube.
A recording of whether the planarian was located in the upper or lower half of the test tube was made after 10 minutes. This procedure was repeated using a different planarian (DugesiaÂ sp.) to allow for collection of independent observations. This time, the test tubeâ€™s position in the holder was reversed, with the stoppered end facing down but still ensuring that there was even illumination of the test tube. This was supposed to act as a control of the fact that only one end of the test tube was stoppered. The location of the planarian in the test tube was again recorded after 10 minutes.
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