Weighing of the rats, as well as the measurement of water and food intake, was done once every week.
The sample was kept under appropriate conditions.
In bio-chemistry, the measure of toxic effect included sodium, potassium, glucose, total cholesterol, creatinine, urea, total albumin and protein, ALT and AST enzyme, GGT, and bile acids (Crofton et al., 1991). Organs such as kidney, liver, and spleen necropsied very fast and the tissues were preserved by appropriate fixation method. Full histopathology examination was extended to the entire rats test. This was done in order to determine their major effects in tissues and more especially on kidney and liver. This was performed on all the blood samples obtained from all the rats, just before killing them.
Prior to blood sampling, the rats underwent an overnight fasting.
The adrenals, liver, kidneys, testes, thymus, epididymides, spleen, brain, and heart of all rats under study were trimmed of any adherent tissue appropriately and weighed immediately after dissection to avoid dying.
All gross lesions were examined, and histopathology performed on tissues and organs that showed effect in the treated groups.
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