The rate of Glycolysis has been claimed to be affected by different factors namely temperature, and concentration of cobalt acetate (Bodner, pp.372-3). The rate-determining steps in the Glycolysis involve the reaction affected by phosphorylase and phosphofructokinase since enzymes are sensitive to temperatures (Hertz et al, pp.340-2). Temperatures affect steps catalyzed by phosphorylase and phosphofructokinase because temperature affects the configuration of the enzymes.
The rate of Glycolysis is determined by measuring the rate of formation of carbon dioxide.
Aims of the experiment
The experiment sought to determine the influence of temperature on the rate of Glycolysis.
Hypothesis for testing
- Hypothesis 1: increase in temperature increases the rate of Glycolysis hence low temperature is a limiting factor to the rate of glycolysis
- Hypothesis 2: temperature changes do not affect the rate of Glycolysis if the rate of Glycolysis is independent of temperature hence temperature is not a limiting factor.
Reagents required for Glycolysis
- 50 grams of glucose
- 775 grams ofÂ Saccharomyces cerevisiae
- liter equivalent of distilled water
- Four sets of water bath
- Four 50 ml test tubes
The procedure of the experiment
The substrate solution was prepared by mixing 50 grams of glucose (six-carbon sugar) and 775 grams ofÂ Saccharomyces cerevisiae to form one liter of solution. 20 milliliters of the solution were pipetted into each of the 50-milliliter test tubes. The four test tubes were then placed under different thermo-regulated conditions in a water bath. The water baths were labeled as control, cod, warm and hot.
The test tubes were acclimatized by being put into the water bath for five minutes. Any carbon dioxide that had built up during the acclimatization period was got rid of by tapping the test tubes.
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