Large organic polymers, which are constituents of biomass, ought to be broken down into smaller constituents that will increase the surface area to volume ratio. This enables anaerobic bacteria to access the energy potential of the material in use. The breakdown of the large polymers and dissolution of the minuscule molecules is called hydrolysis. Acetate and hydrogen are the products of hydrolysis in the first phase and can be utilized by the methanogens as they are in the intermediate forms. However, volatile fatty acids-VFAs (longer than acetate) need to be catabolized that can be readily used by the methanogens. Elements from the first phase are further broken down by acidogenic or fermentative bacteria during acidogenesis (second phase). Alongside the production of VFAs, â€œcarbon dioxide, ammonia, hydrogen sulfide, and related by-products are also producedâ€ (Khopkar, 2004). This second stage in the production of methane (acidogenesis) is likened to the souring of milk.
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