â€œThe experimentation scientifically evaluated the performance characteristics of two culture-based chromogenic media that are cost-effective for VRE surveillanceâ€ (3, 4). The outcome of the results was based on three parameters that facilitated the analysis. These observable indicators were as follows, Selectivity, Stability and Precision.
Materials and Methods include Chromogenic Media CHM-Gn â€œAâ€ and â€œBâ€ obtained from two different manufacturers, Clinical Stool Specimens(Sterile Collected), VRE strains from culture collections (Table A) and Others: Microscopes, Saline, Agar Plates, Tubes, Incubator Inoculation of allÂ EnterococcusÂ species into two sets of media for
Selectivity determination. For control monitoring, positive control strains from VRE reference (n=4) from two different sources were also cultured. A dilution of twoÂ Enterococcus spp,Â Enterococcus faeciumÂ n=7; and Enterococcus faecalisÂ ;n=2) reference and streaking on unadulterated and assorted cultures of both Media, followed by a 48-hour incubation. Comparative testingâ€(3). An enrichment step was incorporated for stool culture.
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