In 1859 Louis Pasteur completely discounted spontaneous generation theory and brought out a new theory that microbes are the agents that bring about the chemical changes. His main contributions were as follows: chemical changes like fermentation from the vital activities of yeasts and bacteria, a demonstration that air contains micro-organisms, the discovery of anaerobic life and terms aerobic and anaerobic, and most important discovery of causes of cholera and silkworm disease and immunity in sheep against anthrax. Pasteur also proposed the germ theory in 1862 that germs cause infections. He used swan-necked flasks for his inventions. His specially designed curvature of the flasks prevented microbes to reach the broth inside the flasks and there was no growth. Once the neck was broken off, the broth developed bacterial growth. Robert Koch was a pioneer in identifying the germs outside the infected animals, particularly anthrax, in pure culture in 1867. He developed a series of procedures â€“ Kochâ€™s postulates, by which a specific organism could be related to a specific disease.
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