N-linked glycans as the rest of glycans have to be attached to proteins and for the und on cell surface; the glycans under consideration are derived from a core14-sugar element and has to be attached to the group of nitrogen characterized by asparagine. This group is also known as the R-group. As a rule, N-linked glycans perform the functions connected with protein trafficking and hold the most important protein sensors. This is why these glycans promote the increase of stability and restriction of flexibility. To analyze better how the glycans work and cooperate with other components of post-translational modifications, it is offered to rely on the basics of ESI mass spectrometry and the possibilities of electrospray ionization of substances.
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