Using a sterilized pipette, a drop of paramecium culture medium was placed on a glass slide. 10 Î¼l of visco-elastic resin was added and mixed with the culture medium using a toothpick. A slide cover slip was placed on top and then the slide was observed under bright-field microscopy at the different magnifications of the microscope. A sketch of the image observed was made of drawing paper. The slide was then observed under the phase-contrast microcopy and a sketch diagram was made on a piece paper.
Preparation of stained slide
Using a sterilized pipette, a drop of paramecium culture medium was placed on a glass slide. 10 Î¼l of visco-elastic resin was added and mixed with the culture medium using a toothpick. A slide cover slip was placed on top and crystal blue stain added on one edge of the cover slip. Using a piece of â€œKimwipeâ€, the stain solution was drawn into the wet mount. The prepared slide was observed under bright â€“field microscopy and the observed microscopic image was drawn. Similarly, the slide was also observed under the phase-contrast microscopy and the microscopic observed structured of the paramecium was drawn on a paper.
Examination of plant cell
Elodea leave was obtained from the plant body and placed on a clean glass slide. A drop of distilled water was added and a cover slip placed on top. The slide was then observed under the microscope using the bright-field microscopy. A clear diagram of the observed microscopic image was drawn
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