TheÂ FtsZÂ gene encodes for a protein that plays a role in mitochondrial cell division in bacteria such asÂ E. coliÂ (Birnboim & Doly 2001). The encoded protein, a GTPase, resembles eukaryotic tubulins in both function and structure. The aim of this practical was to isolate the FtsZ gene from a donor plasmid (pProEX) and ligate it into a second recipient plasmid, pBluescript KS II (pBKS II). This process, which involves excising a specific fragment of DNA (foreign genetic material) from a genome and ligating it into a plasmid, is called recombinant gene technology. It was first done in the mid-1970s. Common sources of template DNA for recombinant gene technology include cDNA and genomic DNA.
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