Accurate drawings to depict the overall organization of the genusÂ RosaÂ specimen were made before dissecting the plant. The cellular organization of various plant parts was then determined by dissecting the specific plant parts, staining them appropriately and observing them under Ã—10 objective lens of the microscope. The surface of epidermal peels was coated with nail polish, allowed to dry and mounted on a slide. The slide was then observed to determine the abundance and distribution stomata as well as other leaf surface features. A thin section of the specimen stem was stained with phloroglucinol-HCl stain and observed under the microscope. For the toluidine blue test (TBO), fresh sections of the stem were mounted in 0.05% TBO in water for a maximum of 1 minute and rinsed in water for an additional minute. The stained stems were then mounted in water.
For the iodine staining, fresh sections of the stem and root were dipped in iodine solution comprising 0.5% iodine in 5% aqueous KI for 2 minutes followed by rinsing with water and mounting in water. Sudan IV staining was done by fixing petal sections in 10% formalin for 10 minutes after which the formalin was replaced with 50% ethanol for
about 1 minute. The fixed sections were then stained with Sudan IV dye saturated in 70% ethanol for 30 minutes followed by rinsing with 50% ethanol and mounting in 75% glycerol.
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