A potato, which contained amylase, was sliced into tiny squares of approximately 4 to 5 millimeters wide. A mark was made about 3 centimeters from the bottom of a large test tube. Subsequently, the pieces of potato were added just below the mark. Several such test tubes were prepared and kept ready for further investigations.
To establish the effect of temperature on the enzyme catalase, three Erlenmeyer flasks were filled halfway with water and placed at different temperatures. The first flask was placed in the refrigerator at less than 10Â oC, whereas the second one was placed at 40Â oC on a hot plate. The third flask, on the other hand, was placed on a hot plate until it attained a temperature of 80Â oC. About 5 ml of 3% hydrogen peroxide was added to three test tubes in the water baths. The test tubes containing the H2O2Â were put in the water baths and allowed to incubate until they attained the required temperatures. The cold, warm and hot hydrogen peroxide was added to the potato squares up to the 6 cm mark after which the height of bubbles was measured for each temperature.
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